Visualization of effector protein translocation from Agrobacterium tumefaciens into host cells

Abstract

In our research protein translocation from Agrobacterium

into yeast and plant cells is studied to obtain fundamental insights in the

translocation process and in the fate of the translocated proteins in the

host cells and the potential biotechnological applications of Agrobacterium mediated protein translocation were explored. In this thesis we studied the Agrobacterium virulence protein expression, translocation and localization via direct visualization and also potential biotechnological applications of protein translocation from Agrobacterium into the recipient cells.

Agrobacterium tumefaciens Agrobacterium mediated transformation Effector Protein Translocation Protein Translocation Visualization Split GFP System PhiLOV2.1 Flourecent Protein Protein Translocation Application