Advancing zebrafish models in proteomics

Author(s):
Duivesteijn, S.J.
Published
2018
Publication type
Doctoral Thesis

Abstract

This thesis

describes the introduction of the zebrafish animal model to the field of

proteomcis. The work presented provided the research community with the first

zebrafish spectral library, which is an important first step to introduce zebrafish to proteomics research. This thesis also provides proteomics resourses to automate and simplify the creation of spectral libraries. Furthermore, it describes the use of the software programme CompareMS2 to provide information on similarities across proteomics databases. In addition, the programme has shown to be applicable as a quick first analysis step on large proteomics datasets. Besides, the thesis demonstrates the advantages and applicability of a multidisciplinary approach, addressing multiple omics, exemplified by using two closeley related model species (zebrafish and common carp). Finally, an overview is given on the advantages and disadvantages of the zebrafish animal model within the field of proteomics. The practical approach to zebrafish models in proteomics described in this thesis resulted in experimental protocols, a standard spectral library and data analysis tools, and significantly contributed to maturing of the zebrafish animal model system for proteomics research.

ProteomicsZebrafishZebrafish modelsSpectral library
Defense date
2018-10-30
Language
English
Supervisor(s)
Deelder, A.M.; Meijer, A.H.
Advisor(s)
Palmblad, N.M.
Committee member(s)
Prins, J.B.; Ottenhoff, T.H.M.; Spaink, H.P.; Verbeek, F.J.; Sar, A. van der
Qualification
Ph.D.
Degree granting institution
Institute of Biology (IBL), Faculty of Science, Leiden University, Leiden
hdl
1887/66790
ISBN(s)
9789463750578

Funding

Sponsorship
The work described in this thesis was was funded by the Netherlands Organization for Scientific Research (NWO), Vidi grant 917.11.398