CRISPR/Cas-induced targeted mutagenesis with Agrobacterium mediated protein delivery

Author(s):
Schmitz, D.J.
Published
2018
Publication type
Doctoral Thesis

Abstract

The RNA guided endonuclease based on the CRISPR/Cas system

of Streptococcus pyogenus is a potent new tool for genome engineering in

plants. Delivery of this system is usually done by transforming plants (and

other organisms) with Agrobacterium tumefaciens which transfers a T-DNA encoding the required genes through its Type IV Secretion System (T4SS). Instead, here we report the delivery of the Cas9 protein of the CRISPR/Cas system of Streptococcus pyogenus through the T4SS of Agrobacterium to Nicotiana benthamiana and Saccharomyces cerevisiae.

Type IV secretion systemlT4SSAgrobacteriumCRISPR/CasTargete mutagenesisProtein translocation
Defense date
2018-09-20
Language
English
Supervisor(s)
Hooykaas, P.J.J.
Advisor(s)
Pater, B.S. de
Committee member(s)
Vergunst, A.C.; Memelink, J.; Spaink, H.P.; Otten, L.
Qualification
Ph.D.
Degree granting institution
Institute of Biology, Faculty of Science, Leiden University, Leiden
hdl
1887/65634