Activity-based proteomics of the endocannabinoid system

Author(s):
Rooden, E.J. van (0000-0003-0041-6455)
Published
2018
Publication type
Doctoral Thesis

Abstract

This thesis describes the

use of an activity-based proteomics method to study the endocannabinoid system.

A protocol for label-free chemical proteomics to measure serine hydrolase

activity in mouse tissue is described. This method is used to compare a Niemann-Pick Type C mouse model to healthy mice. Additionally, several novel activity-based probes for the enzymes diacylglycerol lipase alpha and a/b-hydrolase domain containing protein 6 are described.

Activity-based protein profilingProteomicsEndocannabinoid systemActivity-based probesNiemann-Pick type cLabel free quantificationQuenched activity-based probeBio orthogonal chemistry
Defense date
2018-09-11
Language
English
Supervisor(s)
Stelt, M. van der; Overkleeft, H.S.
Committee member(s)
Aerts, J.M.F.G.; Brouwer, J.; Dekker, F.J.; Marel, G.A. van der; Willems, L.
Qualification
Ph.D.
Degree granting institution
Institute of Chemistry, Faculty of Science, Leiden University, Leiden
hdl
1887/65174