Activity-based proteomics of the endocannabinoid system

Rooden, E.J. van (2018)

Doctoral Thesis

This thesis describes the use of an activity-based proteomics method to study the endocannabinoid system. A protocol for label-free chemical proteomics to measure serine hydrolase activity in mouse tissue is described. This method is used to compare a Niemann-Pick Type C mouse model to healthy mice. Additionally, several novel activity-based probes for the enzymes diacylglycerol lipase alpha and a/b-hydrolase domain containing protein 6 are described.

• Activity-based protein profiling
• Proteomics
• Endocannabinoid system
• Activity-based probes
• Niemann-Pick type c
• Label free quantification
• Quenched activity-based probe
• Bio orthogonal chemistry

• All authors: Rooden, E.J. van
• Supervisor: Stelt, M. van der; Overkleeft, H.S.
• Committee: Aerts, J.M.F.G.; Brouwer, J.; Dekker, F.J.; Marel, G.A. van der; Willems, L.
• Qualification: Doctor (dr.)
• Awarding Institution: Institute of Chemistry, Faculty of Science, Leiden University
• Date: 2018-09-11