Total synthesis of alginate and zwitterionic SP1 oligosaccharides

Abstract

The work in this thesis has been focused on two subjects.

The first is the assembly of alginate oligosaccharides and the generation of

building blocks for the enzymatic synthesis of alginate, and the second is the

total synthesis of large fragments of the zwitterionic SP1 polysaccharide. With these fragments, details about alginate biosynthesis can be obtained through binding studies with biosynthesis enzymes, conjugate vaccines can be generated and binding studies with major histocompatibility complex II molecules can be studied.