Determinants of genome editing outcomes: the impact of target and donor DNA structures

Author(s):
Chen, X.
Published
2018
Publication type
Doctoral Thesis

Abstract

By investigating the interaction between different types of nucleases (i.e. nicking versus cleaving), donor DNA structures and target chromatin environments, this thesis provides important insights into how to improve the three crucial parameters of genome editing: efficiency, specificity and fidelity. The work presented in this thesis expand the range of possibilities for high-fidelity genetic manipulation of human cells.

CRISPRCRISPR-Cas9Genome editingChromatin impactGenome engineeringOff-target
Defense date
2018-05-16
Language
English
Supervisor(s)
Hoeben, R.C.
Advisor(s)
Gonçalves, M.A.F.V.
Committee member(s)
Tijsterman, M.; Schambach, A.; Maarel, S.M. van der; Kühn, R.; Ressing, M.E.
Qualification
Ph.D.
Degree granting institution
Cell and Chemical Biology, Medicine / Leiden University Medical Center (LUMC), Leiden University, Leiden
hdl
1887/62204
ISBN(s)
9789463323499