Environmental and molecular regulation of phenazine-1-carboxamide biosynthesis in Pseudomonas chlororaphis strain PCL1391

Abstract

The biocontrol strain studied in this thesis is Pseudomonas chlororaphis strain PCL1391, which controls tomato foot and root rot caused by Fusarium oxysporum f. sp. radicis-licopersici (Chin-A-Woeng et al., 1998). Production of the anti-fungal metabolite phenazine-1-carboxamide (PCN) and an efficient root colonizing ability (Chin-A-Woeng et al., 2000) are crucial for efficient biocontrol by P. chlororaphis strain PCL1391. This thesis aims at the identification and analysis of environmental conditions affecting the production of PCN and the identification of novel genes involved in the regulation of PCN production.