Dynamics of a GPCR studied with single-molecule microscopy

Author(s):
Keijzer, Sandra de
Published
2006
Publication type
Doctoral Thesis

Abstract

The behavior of single G-protein coupled receptor molecules were studied with single-molecule microscopy in the plasmamembrane during Dictyostelium discoideum chemotaxis. The mobility of the receptor was different in the anterior and posterior regions of living cells migrating towards the source of chemoattractant. This difference of mobility can account for an amplification of the extracellular signal, necessary for chemotaxis. It was also found that the receptor molecules are internalized.

Single-molecule microscopyChemotaxisFluorescent proteinsG-protein coupled receptorDictyostelium discoideumMobility
Defense date
2006-04-12
Language
English
Supervisor(s)
Schmidt, T.; Spaink, H.P.
Advisor(s)
Snaar-Jagalska, B.E.
Qualification
Ph.D.
Degree granting institution
Leiden Institute of Physics, Faculty of Science, Leiden University, Leiden
hdl
1887/4363
ISBN(s)
9090205438

Funding

Sponsorship
NWO; Newport Spectra Physics and Zeiss