On fetomaternal hemorrhage

Pelikan, D.M.V. (2006)

Doctoral Thesis

In this thesis we focused on microscopic and flow cytometric methods suitable to quantify fetal red cells in maternal blood. We compared several methods for FMH quantification both in spiked samples and samples from pregnant women following invasive procedures and complications in pregnancy. We developed a technique for automated read-out of Kleihauer-Betke stained slides to enumerate fetal red cells in maternal blood. We studied the biological behaviour of fetal red and mononuclear cells (MNCs) in the maternal circulation after large FMH and the rate by which these cells are cleared from the maternal circulation. We identified fetal MNCs using a staining with a monoclonal antibody (mAb) directed against the paternally inherited HLA-A2. Furthermore, we discuss the importance of a correct use of the formula to calculate the FMH volume.

• Fetomaternal hemorrhage
• Fetal cells
• Fetal erythrocytes
• Fetal mononuclear cells
• Kleihauer-Betke test
• Automated microscopy
• Flow cytometry
• Microchimerism
• Chorionic villus sampling
• Fetal hemoglobin

• All authors: Pelikan, D.M.V.
• Supervisor: Kanhai, H.H.H.; Tanke, H.J.; Scherjon, S.A.
• Co-supervisor: Scherjon S.A.| Referent: Surbek D.
• Qualification: Doctor (dr.)
• Awarding Institution: Faculty of Medicine, Leiden University Medical Center (LUMC), Leiden University
• Date: 2006-03-23
• Publisher: Den Haag: Pasmans Offsetdrukkerij B.V.
• ISBN (print): 9090204342
• Court: LEI Universiteit Leiden
• Sponsorship: Supported by the Netherlands Organization for Health Research and Development (ZonMw); grant no. 920-03-120