On fetomaternal hemorrhage

Author(s):
Pelikan, D.M.V.
Published
2006
Publication type
Doctoral Thesis

Abstract

In this thesis we focused on microscopic and flow cytometric methods suitable to quantify fetal red cells in maternal blood. We compared several methods for FMH quantification both in spiked samples and samples from pregnant women following invasive procedures and complications in pregnancy. We developed a technique for automated read-out of Kleihauer-Betke stained slides to enumerate fetal red cells in maternal blood. We studied the biological behaviour of fetal red and mononuclear cells (MNCs) in the maternal circulation after large FMH and the rate by which these cells are cleared from the maternal circulation. We identified fetal MNCs using a staining with a monoclonal antibody (mAb) directed against the paternally inherited HLA-A2. Furthermore, we discuss the importance of a correct use of the formula to calculate the FMH volume.

Fetomaternal hemorrhageFetal cellsFetal erythrocytesFetal mononuclear cellsKleihauer-Betke testAutomated microscopyFlow cytometryMicrochimerismChorionic villus samplingFetal hemoglobin
Defense date
2006-03-23
Language
English
Supervisor(s)
Kanhai, H.H.H.; Tanke, H.J.; Scherjon, S.A.
Advisor(s)
Scherjon S.A.| Referent: Surbek D.
Qualification
Ph.D.
Degree granting institution
Faculty of Medicine, Leiden University Medical Center (LUMC), Leiden University, Leiden
Publisher
Den Haag: Pasmans Offsetdrukkerij B.V.
hdl
1887/4347
ISBN(s)
9090204342

Juridical information

Court
LEI Universiteit Leiden

Funding

Sponsorship
Supported by the Netherlands Organization for Health Research and Development (ZonMw); grant no. 920-03-120