Maskless photolithography for rapid Organ-on-a-Chip prototyping and microvascular engineering

Author(s):
Kasi, D.G.
Published
2025
Publication type
Doctoral Thesis

Abstract

This thesis describes facile maskless photolithography-based methods to rapidly prototype Organ-on-a-Chip devices in a cleanroom-free fashion, engineer microvasculature on-chip and the development of substrates suitable for reference-free traction force microscopy. Moreover, the generation and characterization of patient-derived human induced pluripotent stem cells (hiPSCs) and subsequently derived endothelial cells is described.

Organ-on-a-ChipMaskless photolithographyMicrovasculatureEngineeringPrototypingCleanroom-freeDisease modelingIn vitro modelsDevelopmentAnimal-free
Defense date
2025-12-11
Language
English
Supervisor(s)
Maagdenberg, A.M.J.M. van den
Advisor(s)
Orlova, V.V.
Committee member(s)
Maarel, S.M. van der; Goumans, M.J.T.H.; Meer, A.D. van der; Maassen van den Brink, A.
Qualification
Ph.D.
Degree granting institution
Faculty of Medicine, Leiden University Medical Center (LUMC), Leiden University, Leiden
hdl
1887/4285618
ISBN(s)
9789465229393

Funding

Sponsorship
This work was supported by the following grants: The Netherlands Organ-on-Chip Initiative (NOCI), an NWO Gravitation project (024.003.001), the LymphChip project (NWA-ORC 2019 1292.19.019) and The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW) which is supported by a Novo Nordisk Foundation grant (NNF21CC0073729). In addition, financial support for printing of this thesis was provided by Stichting Proefdiervrij.