hiPSC-derived 3D cardiac microtissue models with integrated immune cells and vasculature

Arslan-van Bergen, U. (2024)

Doctoral Thesis

This PhD thesis by Ulgu Arslan titled as “hiPSC-derived 3D cardiac microphysiological systems that can integrate immune and vascular components” explores culture conditions and their effects to incorporate human induced pluripotent stem cell derived macrophages in 3D cardiac microtissues; and endothelial cells to form vascularized and perfusable 3D cardiac microtissues on chips. It discusses and compares current state-of-the-art 3D microphysiological models with respect to their applications, readouts, limitations and possible implementation for their future use. Then, it establishes static microtissue or perfused organ-on-chip models with an emphasis on their potential to study microenvironmental cues, cellular crosstalk, disease modelling and drug testing in cardiovascular research.

• Cardiovascular research
• Heart-on-chip
• Cardiac microtissues
• hiPSC
• Macrophages
• Vasculature

• All authors: Arslan-van Bergen, U.
• Supervisor: Mummery, C.L.
• Co-supervisor: Orlova, V.
• Committee: Hiemstra, P.S.; Goumans, M.J.T.H.; Loskill, P.; Meer, A.D. van der
• Qualification: Doctor (dr.)
• Awarding Institution: Leiden University Medical Center (LUMC), Leiden University
• Date: 2024-09-24
• ISBN (print): 9789465061856
• Sponsorship: The research described in this thesis as well as its publications was supported by the following grants: the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 812954; The Novo Nordisk Foundation Center for Stem Cell Medicine is supported by Novo Nordisk Foundation grants (NNF21CC0073729).