Spatially resolved sampling of the human oral cavity for metabolic profiling

Author(s):
Ciurli, A. (0000-0002-8550-5379); Derks, R.J.E. (0000-0002-8920-7133); Liebl, M.; Ammon, C.; Neefjes, J.; Giera, M.
Published
2021
Publication type
Article / Letter to editor

Abstract

Here, we present a spatially resolved sampling protocol for the oral human cavity aimed at untargeted metabolomics. We describe the spatial collection of salivary biospecimens, their preparation, and subsequent mass-spectrometry-based untargeted metabolomics analysis. Our protocol avoids complex procedures generally required for gland-specific saliva collection. For the human oral cavity, we provide an easy, flexible, and reproducible solution to comprehensively map the highly heterogeneous environment and elucidate the functionality of salivary components.

Date issued
2021-12-17
Language
English
Is part of
STAR Protocols (2021), No. 4
hdl
1887/3769459
doi
10.1016/j.xpro.2021.101002