Fluorescent Nuclei Measurements Macro (FNMM), a tool for automated cell quantification in ImageJ

Abstract

A common requirement, in field of life sciences, is the quantification of specific cells in a given fluorescent microscopy image. ImageJ is a popular tool for researchers to develop custom scripts for automated cell quantification. Validating the quality of cell quantification measurements is a common challenge for such researchers. The Fluorescent Nuclei Measurements Macro (FNMM) was developed to assist researchers performing cell quantification by presenting the results in an intuitive manner whilst simultaneously facilitating processed data collection and measurement assurance.