A Lotus japonicus Nodulation System Based on Heterologous Expression of the Fucosyl Transferase NodZ and the Acetyl Transferase NolL in Rhizobium leguminosarum

Author(s):
Bras, C.P.; Jordá, M.A.; Wijfjes, A.H.M.; Harteveld, M.; Stuurman, N.; Thomas-Oates, J.E; Spaink, H.P. (0000-0003-4128-9501)
Published
2000
Publication type
Article / Letter to editor

Abstract

Heterologous expression of NodZ and NoIL proteins in Rhizobium leguminosarum by. viciae led to the production of acetyl fucosylated lipo-chitin oligosaccharides (LCOs), indicating that the NoIL protein obtained from Mesorhizobium loti functions as an acetyl transferase. We show that the NoIL-dependent acetylation is specific for the fucosyl penta-N-acetylglucosamine species. In addition, the NoIL protein caused elevated production of LCOs, Efficient nodulation of Lotus japonicus by the NodZ/NoIL-producing strain was demonstrated. Nodulation efficiency was further improved by the addition of the ethylene inhibitor L-alpha-(2-aminoethoxyvinyl) glycine (AVG).

Date issued
2000-04-01
Language
English
Publisher
AMER PHYTOPATHOLOGICAL SOC
Is part of
Molecular Plant-Microbe Interactions (2000), Vol. 13, no. 4, p. 475-479
hdl
1887/3665644
doi
10.1094/MPMI.2000.13.4.475