Lattice filter for processing image data of three-dimensional protein nanocrystals

Author(s):
Genderen, E. van; Li, Y.W.; Nederlof, I.; Abrahams, J.P. (0000-0001-8216-1868)
Published
2016
Publication type
Article / Letter to editor

Abstract

When 300kV cryo-EM images at Scherzer focus are acquired from approximate to 100nm thick three-dimensional protein nanocrystals using a Falcon 2 direct electron detector, Fourier transformation can reveal the crystalline lattice to surprisingly high resolutions, even though the images themselves seem to be devoid of any contrast. Here, it is reported how this lattice information can be enhanced by means of a wave finder in combination with Wiener-type maximum-likelihood filtering. This procedure paves the way towards full three-dimensional structure determination at high resolution for protein crystals.

Date issued
2016-01-01
Language
English
Publisher
INT UNION CRYSTALLOGRAPHY
Is part of
Acta Crystallographica Section D Structural Biology (2016), Vol. 72, p. 34-39
hdl
1887/3620778
doi
10.1107/S205979831502149X