N-terminal heterogeneity of methylamine dehydrogenase from Thiobacillus versutus

Author(s):
Beeumen, J. van; Driessche, G. van; Huitema, F.; Duine, J.A.; Canters, G.W. (0000-0002-8496-2332)
Published
1993
Publication type
Article / Letter to editor

Abstract

The N-terminal processing of MADH from the bacterium T versutus and the N-terminal heterogeneity of the isolated a subunit of the alpha2beta2 protein complex was demonstrated by a combination of Edman sequence analysis of an electroblotted band, in situ digested with pyroglutamate aminopeptidase, and accurate mass determination of the homogenous subunit by the technique of electrospray ionisation mass spectrometry. From this study, it appears that the corresponding gene of the alpha subunit contains 395 amino acids and that it is preceded by a leader sequence of 31 residues.

Date issued
1993-10-25
Language
English
Publisher
ELSEVIER SCIENCE BV
Is part of
FEBS Letters (1993), Vol. 333, no. 1-2, p. 188-192
hdl
1887/3618581
doi
10.1016/0014-5793(93)80402-G