Chemical protein synthesis: from nanobodies to activity-based probes

Author(s):
Huppelschoten, Y.
Published
2023
Publication type
Doctoral Thesis

Abstract

In this thesis, solid phase peptide synthesis (SPPS) and chemical protein synthesis are used to synthesize novel tools such as assay-reagents and activity-based probes based on Ubiquitin-like proteins⁠. These probes are used to explore the function and specificity of deconjugation enzymes⁠.

Chemical Protein SynthesisNative Chemical LigationPeptide ChemistryProtein ModificationUbiquitin (like) proteinsSolid Phase Peptide Synthesis
Defense date
2023-04-20
Language
English
Supervisor(s)
Ovaa, H.; Vertegaal, A.C.O.
Advisor(s)
Heden van Noort, G.J. van der
Committee member(s)
Neefjes, J.J.C.; Nielsen, T.E.; Kasteren, S.I. van; Bonger, K.M.
Qualification
Ph.D.
Degree granting institution
Faculty of Medicine, Leiden University Medical Center (LUMC), Leiden University, Leiden
hdl
1887/3594444
ISBN(s)
9789464830088

Funding

Sponsorship
European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement No 765445 (ITN UbiCODE)⁠.