An ENDOR and ESEEM study of the blue copper protein azurin

Author(s):
Coremans, J.W.A.; Gastel, M. van; Poluektov, O.G.; Groenen, E.J.J. (0000-0001-7530-9211); Blaauwen, T. den; Pouderoyen, G. van; Canters, G.W. (0000-0002-8496-2332); Nar, H.; Hammann, C.; Messerschmidt, A.
Published
1995
Publication type
Article / Letter to editor

Abstract

We report electron-nuclear double-resonance experiments on a single crystal of azurin at 95 GHz and electron-spin-echo envelope-modulation experiments on frozen solutions of azurin and of the H117G mutant at 9 GHz. The hyperfine and quadrupole tensors of the two remote nitrogens of the histidine ligands of copper are assigned and discussed. A third nucleus is found to contribute to the echo-modulation spectrum and this probably concerns an amide nitrogen of the peptide backbone.

Date issued
1995-03-24
Language
English
Publisher
ELSEVIER SCIENCE BV
Is part of
Chemical Physics Letters (1995), Vol. 235, no. 3-4, p. 202-210
hdl
1887/3590358
doi
10.1016/0009-2614(95)00114-J
URL
https://www.sciencedirect.com/science/article/pii/000926149500114J?via%3Dihub