Two-photon-excited single-molecule fluorescence enhanced by gold nanorod dimers

Author(s):
Lu, X.; Punj, D. (0000-0003-3976-262X); Orrit, M.A.G.J. (0000-0002-3607-3426)
Published
2022
Publication type
Article / Letter to editor

Abstract

We demonstrate two-photon-excited single-mole-culefluorescence enhancement by single end-to-end self-assembledgold nanorod dimers. We employed biotinylated streptavidin asthe molecular linker, which connected two gold nanorods in end-to-end fashion. The typical size of streptavidin of around 5 nmseparates the gold nanorods with gaps suitable for the access offresh dyes in aqueous solution, yet small enough to give very hightwo-photonfluorescence enhancement. Simulations show thatenhancements of more than 7 orders of magnitude can be achievedfor two-photon-excitedfluorescence in the plasmonic hot spots.With such high enhancements, we successfully detect two-photon-excitedfluorescence for a common organic dye (ATTO 610) at the single-molecule, single-nanoparticle level

Date issued
2022-05-16
Language
English
Publisher
AMER CHEMICAL SOC
Is part of
Nano Letters (2022), Vol. 22, no. 10, p. 4215-4222
hdl
1887/3515103
doi
10.1021/acs.nanolett.2c01219