Monitoring the immune responses to vaccination and pertussis: bordetella pertussis and beyond

Author(s):
Diks, A.M.
Published
2022
Publication type
Doctoral Thesis

Abstract

In this thesis, the monitoring of the immune system in balance and during active responses (by flow cytometry) plays a central role. In chapter 2.1 and 2.2, we investigated the optimal sample logistics for high-dimensional flow cytometry in clinical trials. In chapter 3.1-3.4, we monitored the longitidinal kinetics of circulating immune cells in humans after vaccination or bacterial challenge against Bordetella pertussis. Lastly, in chapter 4, we investigated the immune system in humans carrying a genetic variant of PLCG2 'p.P522R', which is associated with increased longevity and reduced chance of developing dementia.

Bordetella pertussisTdapHuman Challenge ModelPlasma cellsVaccinationPhospholipase C gamma 2AlzheimerFlow CytometryPLCG2 rs72824905clinical trial
Defense date
2022-12-21
Language
English
Supervisor(s)
Dongen, J.J.M. van
Advisor(s)
Berkowska, M.A.
Committee member(s)
Orfao, A.; Roestenberg, M.; Bos, N.A.; Holstege, H.
Qualification
Ph.D.
Degree granting institution
Faculty of Medicine, Leiden University Medical Center (LUMC), Leiden University, Leiden
hdl
1887/3503582