Chemical synthesis of fragments of galactosaminogalactan and pel polysaccharides

Zhang, Y. (2021)

Doctoral Thesis

The work described in this Thesis is focused on the assembly of oligosaccharide fragments derived from a fungal polysaccharide, galactosaminogalactan (GAG) and fragments of the exopolysaccharide Pel, generated by Pseudomonas aeruginosa. To assemble the corresponding oligosaccharides, synthetic methodologies, enabling the stereoselective construction of the required cis-glycosidic linkages has been developed. These synthetic fragments will be valuable tools to elucidate the biosynthesis of GAG and Pel, and characterize the enzymes involved therein.

• Galactosaminogalactan
• 1,2-cis-glycosylation
• Aspergillus fumigatus
• Pseudomonas aeruginosa
• Di-tert-butylsilylene (DTBS)-directed α-glycosylation methodology

• All authors: Zhang, Y.
• Supervisor: Codée, J.D.C.; Marel, G.A. van der
• Committee: Overkleeft, H.S.; Boons, G.J.; Bonnaffé, D.; Artola, M.; Filippov, D.V.
• Qualification: Doctor (dr.)
• Awarding Institution: Leiden Institute of Chemistry (LIC), Faculty of Science, Leiden University
• Date: 2021-11-09