Repeat UVA exposure of human skin fibroblasts induces both a transitionary and recovery DNA methylation response

Author(s):
Tilburg, J.; Slieker, R.C. (0000-0003-0961-9152); Suchiman, H.E.D.; Heath, A.; Heemst, D. van; Slagboom, P.E.; Gruijl, F.R. de; Gunn, D.A.; Heijmans, B.T. (0000-0001-5918-0534)
Published
2020
Publication type
Article / Letter to editor

Abstract

Aim: UVA radiation drives skin photoaging in the dermis, plausibly via persistent changes to DNA methylation in dermal fibroblasts. Methods: Genome-wide DNA methylation changes after five repeated daily UVA doses were determined at 48 h (transitionary) and 1 week (recovery) post final irradiation. Results: Differential methylation was found at the transitionary time point in active chromatin states near genes that are highly expressed in fibroblasts and are involved in cellular defensive mechanisms; the majority of these methylation differences were restored to control levels after 7 day recovery. At the recovery time point, new differential methylation occurred at repressed regions near developmental genes, normally weakly expressed in fibroblasts. Conclusion: UVA irradiation induces transitionary and recovery-associated DNA methylation responses in fibroblasts with contrasting functional characteristics.

dermisfibroblastsgenome-wide DNA methylationphotoagingskin agingUVA irradiation
Date issued
2020-04-01
Language
English
Publisher
FUTURE MEDICINE LTD
Is part of
Epigenomics (2020), Vol. 12, no. 7, p. 563-573
hdl
1887/3182201
doi
10.2217/epi-2019-0251
URL
https://www.futuremedicine.com/doi/10.2217/epi-2019-0251