The resting oxidized state of small laccase analyzed with paramagnetic NMR spectroscopy

Author(s):
Dasgupta R.; Sai Sankar Gupta K.B.; Groot H.J.M. de; Ubbink M.
Published
2021
Publication type
Article / Letter to editor

Abstract

nuclei. Two sequence-specific assignments are proposed on the basis of a second-coordination shell variant that also lacks the copper ion at the T1 site, SLAC-T1D/Q291E. This double mutant is found to be exclusively in the RO state, revealing a subtle balance between the RO and the NI states.

Date issued
2021-03-08
Language
English
Is part of
ChemPlusChem (2021), Vol. 22, no. 8, p. 733-740
hdl
1887/3158669
doi
10.1002/cphc.202100063

Funding

Sponsorship
NWO
Grant number
NWO-BOO 022.005.029