Leiden University Scholarly Publications

Persistent URL of this record https://hdl.handle.net/1887/28970

In Collections

This item can be found in the following collections:

Raterink, R.J. (2014)

High-throughput profiling of small molecules using mass spectrometry

Doctoral Thesis
With the persistent efforts to improve healthcare while the costs are ever-increasing, there is a growing demand for healthcare innovations including faster and cheaper analytical methods to support clinical decisions. The in-depth phenotyping of a biological system, such as comprehensive small-molecule profiling, will be key for breakthroughs in biomedical and pharmacological research towards the development of personalized medicine. In this thesis the development of new methods towards high-throughput, mass spectrometry-based profiling of small molecules in complex biological samples is presented. One option for high-throughput mass spectrometric profiling is direct infusion in which no separation techniques are employed prior to MS detection. With the pre-analytical part being a major bottleneck in a typical direct-infusion MS-based analytical workflow, the focus was set on the development of new sample pretreatment and sampling procedures, including miniaturized and automated...Show moreWith the persistent efforts to improve healthcare while the costs are ever-increasing, there is a growing demand for healthcare innovations including faster and cheaper analytical methods to support clinical decisions. The in-depth phenotyping of a biological system, such as comprehensive small-molecule profiling, will be key for breakthroughs in biomedical and pharmacological research towards the development of personalized medicine. In this thesis the development of new methods towards high-throughput, mass spectrometry-based profiling of small molecules in complex biological samples is presented. One option for high-throughput mass spectrometric profiling is direct infusion in which no separation techniques are employed prior to MS detection. With the pre-analytical part being a major bottleneck in a typical direct-infusion MS-based analytical workflow, the focus was set on the development of new sample pretreatment and sampling procedures, including miniaturized and automated concepts with the potential for high-throughput application.Show less
Bioanalysis
Zebrafish
Nanoelectrospray
Metabolomics
Microsampling
Electroextraction
Micro-liquid-liquid-extraction
Sample pretreatment
All authors
Raterink, R.J.
Supervisor
Hankemeier, T.
Co-supervisor
Vreeken, R.J.
Qualification
Doctor (dr.)
Awarding Institution
Faculty of Science, Leiden University
Date
2014-10-01
ISBN (print)
9789074538862