A breached barrier : analysis of stratum corneum lipids and their role in eczematous patients

Smeden, J. van (2013)

Doctoral Thesis

The stratum corneum is the outermost layer of the skin, and acts as the primary barrier against penetration of pathogens, allergens and other exogenous substances into the lower layers of the skin. Crucial for a proper barrier function are the lipids in the stratum corneum, mainly consisting of ceramides, free fatty acids, and cholesterol. These lipids are highly ordered stacked layers. In atopic eczema the skin barrier is impaired, but the exact role of the lipids is still uncertain. This thesis describes the importance of a proper composition and ordering of the stratum corneum lipids and their role in eczematous patients (atopic eczema and Netherton disease). The results demonstrate that patients suffering from a impaired skin barrier also show different lipid profile. In particular the carbon chain length of the lipids is notably reduced in these eczematous patients. Consequently, the stratum corneum lipids show a less ordered lipid arangement. This is the first time that a strong correlation is observed between the lipid composition, the lipid organization, and the skin barrier function. These studies therefore provide new targets for possible drug therapy of eczematous patients.

• Skin barrier function
• Liquid chromatography / Mass spectrometry
• Netherton Syndrome
• Human skin equivalents
• Atopic Eczema
• Lipid organization
• Free fatty acids
• Ceramides
• Stratum corneum lipids

• All authors: Smeden, J. van
• Supervisor: Bouwstra, J.A.
• Co-supervisor: Vreeken, R.J.
• Qualification: Doctor (dr.)
• Awarding Institution: Faculty of Science, Leiden University
• Date: 2013-06-20
• Sponsorship: STW, Astellas, Evonik, RiverD, Unilever