Characterization of oligosaccharides with capillary high performance anion exchange chromatography hyphenated to pulsed amperometric detection and ion trap mass spectrometry : application to the analysis of human lysosomal disorders

Abstract

The development of a capillary ion chromatograph is described together with a matching desalter. This desalter made it possible to use on-line a mass spectrometer. The mass spectrometer enables partly to characterize carbohydrates eluting from the anion exchange column. This separation technology is named cap-HPAEC-PAD-MS. The developed capillary ion chromatograph is successfully used to characterize glycan structures in human body fluids, such as urine and amniotic fluid, from individuals suffering form a lysosomal disorder