The systems biology of MHC class II antigen presentation

Abstract

Major histocompatibility class II molecules (MHC class II) are one of the key regulators of adaptive immunity because of their specific expression by professional antigen presenting cells (APC). They present peptides derived from endocytosed material to T helper lymphocytes. Consequently, MHC class II is fundamental in orchestrating both cellular and humoral immune responses. A genetic association of certain MHC class II alleles with autoimmunity has long been established. The molecular mechanisms underlying this association are only poorly understood. An in depth understanding of the antigen presentation pathway by MHC class II is essential for the improvement of current therapies. In this thesis, the tools to arrive at a systems understanding of MHC class II antigen presentation are discussed. What are the advantages and disadvantages of a genome-wide screen? And how can a multi-dimensional, data-integrating approach increase the understanding of the systems biology of MHC class II?