Gas chromatography mass spectrometry: key technology in metabolomics

Abstract

Metabolomics involves the unbiased quantitative and qualitative analysis of the complete set of metabolites present in cells, body fluids and tissues. Gas chromatography coupled to mass spectrometry (GC-MS) is very suitable for metabolomics analysis, as it combines high separation power with sensitive and selective mass detection. This thesis is dedicated to the development of reliable quantitative GC-MS based methods for metabolomics analysis.