5-Aminosalicylic Acid Interferes in the Cell Cycle of Colorectal Cancer Cells and Induces Cell Death Modes

Author(s):
Koelink, P.J.; Mieremet-Ooms, M.A.C.; Corver, W.E.; Wolanin, K.; Hommes, D.W.; Lamers, C.B.H.W.; Verspaget, H.W. (0000-0001-5769-6887)
Published
2010
Publication type
Article / Letter to editor

Abstract

Introduction: Epidemiological data suggests that 5-aminosalicyclic acid (5-ASA), a nonsteroidal antiinflammatory drug used in the treatment of inflammatory bowel diseases, prevents colorectal cancer development in these patients, although the mechanisms remain incompletely understood. Methods and Results: Here we report that 5-ASA prevents growth of several colorectal cancer cell lines by interfering in the cell cycle, i.e., an S-phase and G2/M phase arrest, dependent on 5-ASA dosage and concentration, together with an increased mitotic index. In addition, prolonged cell cycle arrest by repeated 5-ASA treatment induced apoptosis and caused abnormal spindle organization leading to mitotic catastrophe, another form of cell death. Conclusion: These observations illustrate that 5-ASA has chemopreventive and chemotherapeutic properties.

5-ASA apoptosis mitotic catastrophe cell death colorectal cancer activated-receptor-gamma flow-cytometry mitotic catastrophe carcinoma cells ppar-gamma inhibition apoptosis colon growth risk
Date issued
2010-03-31
Language
English
Is part of
Inflammatory Bowel Diseases (2010), Vol. 16, no. 3, p. 379-389
hdl
1887/109800
doi
10.1002/ibd.21086