Genotyping of DNA Samples Isolated from Formalin-Fixed Paraffin-Embedded Tissues Using Preamplification

Author(s):
Baak-Pablo, R.; Dezentje, V.; Guchelaar, H.J. (0000-0002-7085-1383); Straaten, T. van der
Published
2010
Publication type
Article / Letter to editor

Abstract

DNA isolated from formalin fixed paraffin embedded (FFPE) tissue is often fragmented and cross linked and is therefore difficult to genotype To enable this source of DNA for genotyping analysis using Taqman probes, we tested whether enrichment of the target genes would increase the amount of available DNA For enrichment of the target genes, we used preamplification by means of diluted Taqman assays To establish the appropriateness of preamplification, we used DNA extracted from paraffin embedded tissue and compared the genotyping results of a series of single nucleotide polymorphisms assessed m DNA samples with and without preamplification In a subset of patients, DNA was isolated from both blood and FFPE tissue to test the reliability of genotyping results derived after preamplification We found an increase in call rate after preamplification and a convincing con cordance in genotype Based on our findings, we can safely conclude that preamplification of DNA isolated from paraffin-embedded tissue is a valuable and reliable method to optimize genotyping results (J Mol Diagn 2010, 12 746-749, DOI 10.2353/jmoldx.2010.100047)

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Date issued
2010-11-30
Language
English
Is part of
The Journal of Molecular Diagnostics (2010), Vol. 12, no. 6, p. 746-749
hdl
1887/109586
doi
10.2353/jmoldx.2010.100047