Comparison of methods for the analysis of therapeutic immunoglobulin G Fc-glycosylation profiles-Part 2: Mass spectrometric methods

Author(s):
Reusch, D.; Haberger, M.; Falck, D. (0000-0003-3908-2376); Peter, B.; Maier, B.; Gassner, J.; Hook, M.; Wagner, K.; Bonnington, L.; Bulau, P.; Wuhrer, M. (0000-0002-0814-4995)
Published
2015
Publication type
Article / Letter to editor
IgG glycosylationmonoclonal antibody (mAb)HILIC-UPLCmethod comparisonmass spectrometryESI-MSMALDI-MSLC-MSglycan analysisPNGase FPeptide-N-Glycosidase FmAbmonoclonal antibodyFcfragment crystallizableHILIC-UHPLChydrophilic interaction liquid chromatography-ultra high performance liquid chromatography2-AB2-aminobenzamideFabfragment antigen-bindingIgGimmunoglobulin GPGC-MSporous graphitized carbon chromatography- mass spectrometryelectrospray ionization-mass spectrometryMALDImatrix assisted laser desorption ionizationIdeS proteaseproteolytic enzyme like protease from Streptococcus pyrogenesHPAEC-PADhigh-performance anion exchange chromatography with pulsed amperometric detectionRP-HPLCreversed phase high performance liquid chromatographyCEcapillary electrophoresisTICtotal ion chromatogramLCMSliquid chromatography-mass spectrometry
Date issued
2015-07-04
Language
English
Is part of
mAbs (2015), Vol. 7, no. 4, p. 732-742
hdl
1887/101099
doi
10.1080/19420862.2015.1045173